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Modern Biophysical Chemistry : Detection and Analysis of Biomolecules
This updated and up-to-date version of the first edition continues with the really interesting stuff to spice up a standard biophysics and biophysical chemistry course.All relevant methods used in current cutting edge research including such recent developments as super-resolution microscopy and next-generation DNA sequencing techniques, as well as industrial applications, are explained.The text has been developed from a graduate course taught by the author for several years, and by presenting a mix of basic theory and real-life examples, he closes the gap between theory and experiment. The first part, on basic biophysical chemistry, surveys fundamental and spectroscopic techniques as well as biomolecular properties that represent the modern standard and are also the basis for the more sophisticated technologies discussed later in the book.The second part covers the latest bioanalytical techniques such as the mentioned super-resolution and next generation sequencing methods, confocal fluorescence microscopy, light sheet microscopy, two-photon microscopy and ultrafast spectroscopy, single molecule optical, electrical and force measurements, fluorescence correlation spectroscopy, optical tweezers, quantum dots and DNA origami techniques.Both the text and illustrations have been prepared in a clear and accessible style, with extended and updated exercises (and their solutions) accompanying each chapter. Readers with a basic understanding of biochemistry and/or biophysics will quickly gain an overview of cutting edge technology for the biophysical analysis of proteins, nucleic acids and other biomolecules and their interactions.Equally, any student contemplating a career in the chemical, pharmaceutical or bio-industry will greatly benefit from the technological knowledge presented.Questions of differing complexity testing the reader's understanding can be found at the end of each chapter with clearly described solutions available on the Wiley-VCH textbook homepage under: www.wiley-vch.de/textbooks
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Solvation Effects on Molecules and Biomolecules : Computational Methods and Applications
The incessant development of quantum chemistry since the appearance of the Schrodinger ¨ equation has turned this area into a respectable branch of science with unprecedented capabilities.It is now a well-recognized eld of research with pred- tive power that is an important component in physical–chemical laboratories.Very important developments were conducted in the early days by bright theoretical s- entists that were ready to absorb the incredible and unpredicted computer revolution which was only just beginning.Isolated medium-size molecular systems can now be accurately studied theoretically by quantum chemical methods.However, it was also long recognized that all biomolecular phenomena necessary to obtain and sustain living systems take place in solution, as well as the vast majority of chemical p- cesses.Indeed solvent and liquid systems are germane in chemistry experiments.In physics, aconstant concern isthedescription of theroleplayed by theenvironment in modifying the properties of the system as compared to the isolated situation.Hence, the importance of studying atoms, molecules and biomolecules in the solvent en- ronment can hardly be denied.The quantum chemical studies of molecular systems affected by the interaction with a solvent had its own turning point before the end of the 1970s, when some pioneering work was done, including the dielectric pr- erties of the medium in an effective nonlinear Hamiltonian.This naturally led to the development of the so-called continuum models that are important and now popular.Continuum models can be implemented from the simplest to the most sophisticated quantum chemical methods.
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Chemical Theory and Multiscale Simulation in Biomolecules : From Principles to Case Studies
Chemical Theory and Multiscale Simulation in Biomolecules: From Principles to Case Studies helps readers understand what simulation is, what information modeling of biomolecules can provide, and how to compare this information with experiments.Beginning with an introduction to computational theory for modeling, the book goes on to describe how to control the conditions of modeling systems and possible strategies for time-cost savings in computation.Part Two further outlines key methods, with step-by-step guidance supporting readers in studying and practicing simulation processes.Part Three then shows how these theories are controlled and applied in practice, through examples and case studies on varied applications. This book is a practical guide for new learners, supporting them in learning and applying molecular modeling in practice, whilst also providing more experienced readers with the knowledge needed to gain a deep understanding of the theoretical background behind key methods.
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Nanomaterials-Based Sensing Platforms : Towards the Efficient Detection of Biomolecules and Gases
Sensors are effective tools used to carry out cost-effective, fast, and reliable sensing for a wide range of applications.This volume presents a brief history behind sensing technology and highlights a broad range of biosensing techniques based on optical and electrochemical response methods.Starting from the traditional enzyme-based biosensing method to functionalized nanostructure-based sensors, this book also provides a detailed overview of some of the advanced sensing methodologies based on photonic crystal cavity-based sensing devices. The authors showcase the extraordinary success of nanomaterials, their current strategical exploitation, and an unprecedented pool of possibilities they hold for the future.Many of the technologies have been developed recently for the sensing of various bioanalytes and molecules, some of which have been included in this book through dedicated chapters.The book looks at various sensors, such as for biosensing, electrochemical sensing, gas sensing, photoelectrochemical sensing, and colorimetric sensing, all of which have shown vast potential.
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Rare-Earth Element Biochemistry: Characterization and Applications of Lanthanide-Binding Biomolecules : Volume 651
Rare-Earth Element Biochemistry: Characterization and Applications of Lanthanide-Binding Biomolecules, Volume 651 in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field.Chapters in this new release include Spectrophotometric methods to probe the solution chemistry of lanthanide complexes with macromolecules, Determination of affinities of lanthanide-binding proteins using chelator-buffered titrations, Electron Paramagnetic Resonance of Lanthanides, Characterization of lanthanoid binding proteins using NMR spectroscopy, Macromolecular crystallography for f-element complex characterization, Infrared spectroscopy probes ion binding geometries, Predicting lanthanide coordination structures in solution with molecular simulation, and much more. Additional sections cover the Characteristics of Gd(III) spin labels for the study of protein conformations, Lanthanide-based resonance energy transfer biosensors for live-cell applications, Yttrium-86 PET imaging, Aqueous Chemistry of the Smallest Rare Earth: Comprehensive Characterization of Radioactive and Non-radioactive Scandium Complexes for Biological Applications, and In vitro selection and application of lanthanide-dependent DNAzymes.
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